Filamin A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721188

Recombinant Filamin A protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for Filamin A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2721188)

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This Filamin A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Filamin-A (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Henkels, Mallets, Dennis, Gomez-Cambronero: "S6K is a morphogenic protein with a mechanism involving Filamin-A phosphorylation and phosphatidic acid binding." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 4, pp. 1299-313, (2015) (PubMed).

Target Details

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Alternative Name: Filamin-A

Background: The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009].

Molecular Weight: 280.6 kDa

NCBI Accession: NP_001104026

Pathways: TCR Signaling, Maintenance of Protein Location