GIT2 Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721505

This Recombinant GIT2 protein is expressed in HEK-293 Cells.

Quick Overview for GIT2 Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag) (ABIN2721505)

Target: GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 4

Purification tag / Conjugate: This GIT2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4 (transcript variant 4) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Alternative Name: G Protein-Coupled Receptor Kinase Interacting Arfgap 2 (Git2)

Background: This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Molecular Weight: 52.4 kDa

NCBI Accession: NP_631940

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling