GATA3 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721729

This Recombinant GATA3 protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for GATA3 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2721729)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This GATA3 protein is labelled with Myc-DYKDDDDK Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human GATA3 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Biological Activity Comment: GATA3 Activity Verified in a DNA-binding Assay: DNA binding activity of GATA3 as a function of protein concentration. Sensitivity to competitor oligonucleotide demonstrates specificity of protein-DNA binding.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Kao, Stankovic: "Transactivation of human osteoprotegerin promoter by GATA-3." in: Scientific reports, Vol. 5, pp. 12479, (2015) (PubMed).

Target Details

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: Gata3

Background: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.

Molecular Weight: 47.7 kDa

NCBI Accession: NP_002042

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation