GCK Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
Quick Overview for GCK Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2721957)
Target
See all GCK ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- This GCK protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Glucokinase / Hexokinase-4 (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)
custom-made GCK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)custom-made GCK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (GST tag)GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) proteinGCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- GCK (Glucokinase (Hexokinase 4) (GCK))
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Alternative Name
- Glucokinase,hexokinase-4
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Background
- Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
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Molecular Weight
- 51.9 kDa
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NCBI Accession
- NP_277043
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Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
Target
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