Hexokinase 1 Protein (HK1) (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722677

This Recombinant Hexokinase 1 protein is produced in HEK-293 Cells.

Quick Overview for Hexokinase 1 Protein (HK1) (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2722677)

Target: Hexokinase 1 (HK1)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This Hexokinase 1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Hexokinase-1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Hexokinase 1 (HK1)

Alternative Name: Hexokinase-1

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Molecular Weight: 102.3 kDa

NCBI Accession: NP_000179

Pathways: Carbohydrate Homeostasis, Warburg Effect