H2AFZ Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722805

Recombinant H2AFZ protein expressed in HEK-293 Cells.

Quick Overview for H2AFZ Protein (Myc-DYKDDDDK Tag) (ABIN2722805)

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This H2AFZ protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Histone H2A.Z protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Alternative Name: Histone h2a.z

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.

Molecular Weight: 13.4 kDa

NCBI Accession: NP_002097

Pathways: Telomere Maintenance