HLA-DOB Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722840

This Recombinant HLA-DOB protein is expressed in HEK-293 Cells.

Quick Overview for HLA-DOB Protein (Myc-DYKDDDDK Tag) (ABIN2722840)

Target: HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This HLA-DOB protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human HLA class II DO beta / HLA-DOB protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))

Alternative Name: Hla Class II Do Beta,hla-Dob

Background: HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Molecular Weight: 30.6 kDa

NCBI Accession: NP_002111

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response