Interleukin enhancer-binding factor 3 (ILF3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2723411

Recombinant Interleukin enhancer-binding factor 3 protein expressed in HEK-293 Cells.

Quick Overview for Interleukin enhancer-binding factor 3 (ILF3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag) (ABIN2723411)

Target: Interleukin enhancer-binding factor 3 (ILF3)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: Myc-DYKDDDDK Tag

Characteristics:

• Recombinant human ILF3 / DRBF (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Interleukin enhancer-binding factor 3 (ILF3)

Alternative Name: Ilf3,drbf

Background: This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014].

Molecular Weight: 95.2 kDa

NCBI Accession: NP_036350

Pathways: M Phase