Insulin Protein (INS) (Myc-DYKDDDDK Tag)
Quick Overview for Insulin Protein (INS) (Myc-DYKDDDDK Tag) (ABIN2723506)
Target
See all Insulin (INS) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Insulin protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Insulin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Insulin (INS) protein
INS Origin: Human Host: Yeast Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Insulin (INS) (AA 1-110) protein (GST tag)INS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin (INS) (AA 25-54), (AA 90-110) (Active) protein (His tag)INS Origin: Mouse Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Insulin (INS)
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Alternative Name
- Insulin
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Background
- After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 9.3 kDa
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NCBI Accession
- NP_000198
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Pathways
- NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Target
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