IL-10 Protein (Myc-DYKDDDDK Tag)
Quick Overview for IL-10 Protein (Myc-DYKDDDDK Tag) (ABIN2723581)
Target
See all IL-10 (IL10) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This IL-10 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Interleukin-10 / IL10 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Interleukin 10 (IL10) (AA 19-178) protein
IL10 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 10 (IL10) (AA 19-178) (Active) proteinIL10 Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 10 (IL10) (AA 19-178) protein (His-Avi Tag)IL10 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 10 (IL10) (AA 19-178) protein (His-Avi Tag,Biotin)IL10 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 10 (IL10) (Active) proteinIL10 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 10 (IL10) (AA 22-178) protein (His tag)IL10 Origin: Hamster Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 10 (IL10) (AA 1-178) protein (GST tag)IL10 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 10 (IL10) proteinIL10 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Interleukin 10 (IL10) (AA 19-178) protein (His tag)IL10 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-10 (IL10) (Interleukin 10 (IL10))
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Alternative Name
- Interleukin-10,il10
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Background
- The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011].
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Molecular Weight
- 18.6 kDa
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NCBI Accession
- NP_000563
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
Target
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