IL-34 Protein (Transcript Variant 1) (His tag)

Catalog No. ABIN2723714

Recombinant IL-34 protein expressed in CHO Cells.

Quick Overview for IL-34 Protein (Transcript Variant 1) (His tag) (ABIN2723714)

Target: IL-34 (IL34) (Interleukin 34 (IL34))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: CHO Cells

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This IL-34 protein is labelled with His tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Interleukin-34 / IL34 (transcript variant 1) protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 20 - 30 ng/ml, corresponding to a specific activity of 3.3 - 5 x 10^4 units/mg, determined by M-NFS-60 cell proliferation induction in a dose dependent manner.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IL-34 (IL34) (Interleukin 34 (IL34))

Alternative Name: Interleukin-34,il34

Molecular Weight: 26 kDa

NCBI Accession: NP_001128572

Pathways: RTK Signaling