IL-4 Protein (Transcript Variant 1)
Quick Overview for IL-4 Protein (Transcript Variant 1) (ABIN2723725)
Target
See all IL-4 (IL4) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin-4 / IL4 (transcript variant 1) protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 2 - 4 ng/ml, corresponding to a specific activity of 2.5 - 5 x 10^5 units/mg, determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner.
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Interleukin 4 (IL4) (AA 25-153) protein
IL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His tag)IL4 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)IL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag)IL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) protein (His tag)IL4 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 4 (IL4) (AA 25-153) protein (mFc Tag)IL4 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 4 (IL4) (AA 25-153) protein (His tag)IL4 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 1 x PBS
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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Alternative Name
- Interleukin-4,il4
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Molecular Weight
- 13.3 kDa
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NCBI Accession
- NP_067258
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Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
Target
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