IRF9 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2723813

Recombinant IRF9 protein expressed in HEK-293 Cells.

Quick Overview for IRF9 Protein (Myc-DYKDDDDK Tag) (ABIN2723813)

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This IRF9 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human IRF9 / ISGF3G protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Alternative Name: Irf9,isgf3g

Background: Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 43.5 kDa

NCBI Accession: NP_006075

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Hepatitis C