KLF4 Protein (AA 1-470) (GST tag)
(2 references)-
- Target See all KLF4 Proteins
- KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-470
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This KLF4 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- KLF4 (Human) Recombinant Protein (P01)
- Sequence
- MAVSDALLPS FSTFASGPAG REKTLRQAGA PNNRWREELS HMKRLPPVLP GRPYDLAAAT VATDLESGGA GAACGGSNLA PLPRRETEEF NDLLDLDFIL SNSLTHPPES VAATVSSSAS ASSSSSPSSS GPASAPSTCS FTYPIRAGND PGVAPGGTGG GLLYGRESAP PPTAPFNLAD INDVSPSGGF VAELLRPELD PVYIPPQQPQ PPGGGLMGKF VLKASLSAPG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAVSSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQELM PPGSCMPEEP KPKRGRRSWP RKRTATHTCD YAGCGKTYTK SSHLKAHLRT HTGEKPYHCD WDGCGWKFAR SDELTRHYRK HTGHRPFQCQ KCDRAFSRSD HLALHMKRHF
- Characteristics
- Human KLF4 full-length ORF ( NP_004226.2, 1 a.a. - 470 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Kruppel-Like Factor 4 (Gut) (KLF4) protein (Myc-DYKDDDDK Tag)
KLF4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Kruppel-Like Factor 4 (Gut) (KLF4) (AA 221-320) protein (GST tag)KLF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Kruppel-Like Factor 4 (Gut) (KLF4) (AA 1-479) protein (His tag)KLF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Kruppel-Like Factor 4 (Gut) (KLF4) (AA 11-395) protein (HIS-CaM)KLF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
Kruppel-Like Factor 4 (Gut) (KLF4) (AA 1-513) protein (Strep Tag)Crystallography grade KLF4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Kruppel-Like Factor 4 (Gut) (KLF4) (AA 1-483) protein (Strep Tag)Crystallography grade KLF4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Kruppel-Like Factor 4 (Gut) (KLF4) proteinKLF4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Delivery of reprogramming factors into fibroblasts for generation of non-genetic induced pluripotent stem cells using a cationic bolaamphiphile as a non-viral vector." in: Biomaterials, Vol. 34, Issue 21, pp. 5336-43, (2013) (PubMed).
: "DLK2 is a transcriptional target of KLF4 in the early stages of adipogenesis." in: Journal of molecular biology, Vol. 417, Issue 1-2, pp. 36-50, (2012) (PubMed).
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: "Delivery of reprogramming factors into fibroblasts for generation of non-genetic induced pluripotent stem cells using a cationic bolaamphiphile as a non-viral vector." in: Biomaterials, Vol. 34, Issue 21, pp. 5336-43, (2013) (PubMed).
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- Target
- KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))
- Alternative Name
- KLF4 (KLF4 Products)
- Synonyms
- EZF Protein, GKLF Protein, Gklf Protein, Zie Protein, KLF4 Protein, biklf Protein, biklf-A Protein, Kruppel like factor 4 Protein, Kruppel-like factor 4 (gut) Protein, Kruppel-like factor 4 Protein, Kruppel-like factor 4 (gut) L homeolog Protein, KLF4 Protein, Klf4 Protein, klf4 Protein, klf4.L Protein
- Background
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Full Gene Name: Kruppel-like factor 4 (gut)
Synonyms: EZF,GKLF - Gene ID
- 9314
- NCBI Accession
- NM_004235
- Pathways
- Peptide Hormone Metabolism, Stem Cell Maintenance
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