LDHB Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2724574

This Recombinant LDHB protein is expressed in HEK-293 Cells.

Quick Overview for LDHB Protein (Myc-DYKDDDDK Tag) (ABIN2724574)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This LDHB protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human LDHB protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: Ldhb

Background: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

Molecular Weight: 36.5 kDa

NCBI Accession: NP_002291

Pathways: Warburg Effect