LDHB Protein (AA 1-334) (His tag)
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- Target See all LDHB Proteins
- LDHB (Lactate Dehydrogenase B (LDHB))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-334
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LDHB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL
- Purity
- > 95 % by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 150 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.
- Top Product
- Discover our top product LDHB Protein
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Lactate Dehydrogenase B (LDHB) (AA 1-334) protein (GST tag)
LDHB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase B (LDHB) (AA 1-334) (Active) protein (His tag)LDHB Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Lactate Dehydrogenase B (LDHB) (AA 2-334) protein (His tag)Crystallography grade LDHB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase B (LDHB) (AA 2-334) protein (His tag)Crystallography grade LDHB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase B (LDHB) (AA 2-334), (full length) protein (His tag)LDHB Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- LDHB (Lactate Dehydrogenase B (LDHB))
- Alternative Name
- Ldhb (LDHB Products)
- Synonyms
- LDH-B Protein, LDH-H Protein, LDHBD Protein, TRG-5 Protein, AI790582 Protein, H-Ldh Protein, Ldh-2 Protein, Ldh2 Protein, ldh-h Protein, ldh2 Protein, ldhb Protein, ldhba Protein, trg-5 Protein, LDHB Protein, LDH-B-A Protein, wu:fa16d07 Protein, wu:fa20c10 Protein, wu:fa96a08 Protein, LDH-B-B Protein, zgc:92882 Protein, lactate dehydrogenase B Protein, lactate dehydrogenase B S homeolog Protein, lactate dehydrogenase Ba Protein, lactate dehydrogenase Bb Protein, LDHB Protein, Ldhb Protein, ldhb.S Protein, ldhb Protein, ldhba Protein, ldhbb Protein
- Background
- Ldhb, also known as L-lactate dehydrogenase B chain isoform Ldhb, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant mouse Ldhb protein, fused to His-tagging at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 39 kDa (357aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_032518
- UniProt
- P16125
- Pathways
- Warburg Effect
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