LIMK2 Protein (Myc-DYKDDDDK Tag)
This Recombinant LIMK2 protein is expressed in HEK-293 Cells.
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Catalog No. ABIN2724748
$956.40
Plus shipping costs $50.00, if applicable $20.00 dry ice
20 μg
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United States
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Quick Overview for LIMK2 Protein (Myc-DYKDDDDK Tag) (ABIN2724748)
Target
See all LIMK2 Proteins
LIMK2
(LIM Domain Kinase 2 (LIMK2))
Protein Type
Recombinant
Origin
Human
Source
HEK-293 Cells
Application
Antibody Production (AbP), Standard (STD)
Purity
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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Purification tag / Conjugate
- This LIMK2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human LIMK2 (transcript variant 2b) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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LIM Domain Kinase 2 (LIMK2) (AA 1-686) protein (GST tag)
LIMK2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
LIM Domain Kinase 2 (LIMK2) (AA 152-252) protein (GST tag)LIMK2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- LIMK2 (LIM Domain Kinase 2 (LIMK2))
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Alternative Name
- Limk2
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Background
- There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 69.7 kDa
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NCBI Accession
- NP_057952
Target
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