LITAF Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for LITAF Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2724835)
Target
See all LITAF ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This LITAF protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human LITAF (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (AA 1-161) protein (His tag)
custom-made LITAF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (AA 1-161) protein (GST tag)LITAF Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) proteinLITAF Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (AA 1-75) protein (His-IF2DI Tag)LITAF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (AA 1-161) protein (His tag)LITAF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- LITAF (Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF))
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Alternative Name
- Litaf
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Background
- Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.
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Molecular Weight
- 16.9 kDa
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NCBI Accession
- NP_004853
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Pathways
- Cellular Response to Molecule of Bacterial Origin
Target
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