M-CSF/CSF1 Protein (Transcript Variant 3)
Quick Overview for M-CSF/CSF1 Protein (Transcript Variant 3) (ABIN2725103)
Target
See all M-CSF/CSF1 (CSF1) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human M-CSF / CSF-1 (transcript variant 3) protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 2 - 6 ng/ml, corresponding to a specific activity of 1.6 - 5 x 10^5 units/mg, determined by M-NFS60 cell proliferation induced by mouse M-CSF in a dose dependent manner.
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Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) protein
CSF1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) protein (His-Avi Tag)CSF1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (Fc Tag)CSF1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (His tag)CSF1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (His tag)CSF1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190), (Isoform 3) (Active) proteinCSF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by non-reducing SDS-PAGE. Active
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) protein (His-Avi Tag,Biotin)CSF1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-187) (Active) proteinCSF1 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 1 (Macrophage) (CSF1) (Active) proteinCSF1 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (mFc Tag)CSF1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 1 x PBS
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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Alternative Name
- M-Csf,csf-1
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Molecular Weight
- 28.2 kDa
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NCBI Accession
- NP_001107002
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Pathways
- RTK Signaling
Target
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