MASP1 Protein (Transcript Variant 3) (His tag)

Catalog No. ABIN2725534

This Recombinant MASP1 protein is expressed in HEK-293 Cells.

Quick Overview for MASP1 Protein (Transcript Variant 3) (His tag) (ABIN2725534)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This MASP1 protein is labelled with His tag.

Characteristics:

• Recombinant human MASP-1 (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2, pH 8.0

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Alternative Name: Masp-1

Background: This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Molecular Weight: 80.68kD

NCBI Accession: NP_001027019

Pathways: Complement System