MDH1 Protein (Myc-DYKDDDDK Tag)
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- Target See all MDH1 Proteins
- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MDH1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MDH1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MDH1 Protein
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Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) protein (His tag)
MDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) protein (GST tag)MDH1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) protein (His tag)MDH1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by SDS-PAGE
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 2-333), (full length) protein (GST tag)MDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
- Alternative Name
- Mdh1 (MDH1 Products)
- Background
- This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
- Molecular Weight
- 36.2 kDa
- NCBI Accession
- NP_005908
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