MDH1 Protein (His tag)

Catalog No. ABIN7318759

Product Details

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MDH1 protein is labelled with His tag.

Purpose: Recombinant Human MDH1 Protein (His Tag)

Sequence: Ser2-Ala334

Characteristics: Recombinant Human Malate Dehydrogenase, Cytoplasmic is produced by our E.coli expression system and the target gene encoding Ser2-Ala334 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDH1 (MDH1 Products)

Synonyms: MDH-s Protein, MDHA Protein, MGC:1375 Protein, MOR2 Protein, ODS1 Protein, B230377B03Rik Protein, D17921 Protein, Mor-2 Protein, Mor2 Protein, MDL1 Protein, Mdhl Protein, MDH Protein, mdh-s Protein, mdha Protein, mor2 Protein, MDH-1 Protein, Mdh Protein, Mdh-1 Protein, Mdh2 Protein, MdhD Protein, cMdh Protein, sMdh Protein, malate dehydrogenase 1 Protein, malic enzyme 2 Protein, malate dehydrogenase 1, NAD (soluble) Protein, malate dehydrogenase 1 S homeolog Protein, malate dehydrogenase, cytoplasmic Protein, Malate dehydrogenase 1 Protein, MDH1 Protein, ME2 Protein, Mdh1 Protein, mdh1.S Protein, LOC100280767 Protein

Background:

Background: Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.

Synonym: Malate Dehydrogenase Cytoplasmic, Cytosolic Malate Dehydrogenase, Diiodophenylpyruvate Reductase, MDH1, MDHA

Molecular Weight: 37.5 kDa

UniProt: P40925