MED31 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2725746

This Recombinant MED31 protein is produced in HEK-293 Cells.

Quick Overview for MED31 Protein (Myc-DYKDDDDK Tag) (ABIN2725746)

Target: MED31 (Mediator Complex Subunit 31 (MED31))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This MED31 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human MED31 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MED31 (Mediator Complex Subunit 31 (MED31))

Alternative Name: Med31

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 15.6 kDa

NCBI Accession: NP_057144

Pathways: Regulation of Lipid Metabolism by PPARalpha