MED7 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for MED7 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2725751)
Target
See all MED7 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This MED7 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human MED7 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Mediator Complex Subunit 7 (MED7) (AA 1-233) protein (GST tag)
MED7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mediator Complex Subunit 7 (MED7) (AA 1-233) protein (His-IF2DI Tag)MED7 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Mediator Complex Subunit 7 (MED7) (AA 1-233) protein (His tag)MED7 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MED7 (Mediator Complex Subunit 7 (MED7))
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Alternative Name
- Med7
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Background
- The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.
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Molecular Weight
- 27.1 kDa
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NCBI Accession
- NP_004261
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Pathways
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Target
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