MRPS22 Protein (Myc-DYKDDDDK Tag)
Quick Overview for MRPS22 Protein (Myc-DYKDDDDK Tag) (ABIN2726383)
Target
See all MRPS22 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This MRPS22 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human MRPS22 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Mitochondrial Ribosomal Protein S22 (MRPS22) (AA 1-360) protein (His tag)
custom-made MRPS22 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitochondrial Ribosomal Protein S22 (MRPS22) (AA 1-360) protein (GST tag)MRPS22 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MRPS22 (Mitochondrial Ribosomal Protein S22 (MRPS22))
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Alternative Name
- Mrps22
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Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq.
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Molecular Weight
- 41.1 kDa
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NCBI Accession
- NP_064576
Target
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