NCALD Protein (Transcript Variant 1) (His tag)

Catalog No. ABIN2726873

Recombinant NCALD protein expressed in Escherichia coli (E. coli).

Quick Overview for NCALD Protein (Transcript Variant 1) (His tag) (ABIN2726873)

Target: NCALD (Neurocalcin delta (NCALD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This NCALD protein is labelled with His tag.

Characteristics:

• Recombinant human NCALD (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NCALD (Neurocalcin delta (NCALD))

Alternative Name: Ncald

Background: This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein additional variants may exist but their biological validity has not been determined.

Molecular Weight: 24.4 kDa

NCBI Accession: NP_001035714