COBRA1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2727090

This Recombinant COBRA1 protein is produced in HEK-293 Cells.

Quick Overview for COBRA1 Protein (Myc-DYKDDDDK Tag) (ABIN2727090)

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This COBRA1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human NELF-B protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Alternative Name: Nelf-B

Background: NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2 MIM 606026), either NELFC or NELFD (TH1L MIM 605297), and NELFE (RDBP MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1 MIM 603555) and SPT5 (SUPT5H MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Molecular Weight: 65.5 kDa

NCBI Accession: NP_056271