NOL3 Protein (Transcript Variant 3) (His tag)

Catalog No. ABIN2727447

This Recombinant NOL3 protein is produced in Escherichia coli (E. coli).

Quick Overview for NOL3 Protein (Transcript Variant 3) (His tag) (ABIN2727447)

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This NOL3 protein is labelled with His tag.

Characteristics:

• Recombinant human NOL3 / ARC (transcript variant 3) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris-HCl, 1 mM DTT, 1 mM EDTA, 2 mM ß-ME, 20 % Glycerol, pH 7.5. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Alternative Name: Nol3,arc

Background: This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 22.6 kDa

NCBI Accession: NP_001171986