NSMCE2 Protein (His tag)
Quick Overview for NSMCE2 Protein (His tag) (ABIN2727651)
Target
See all NSMCE2 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This NSMCE2 protein is labelled with His tag.
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Characteristics
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- Recombinant human NSMCE2 (full length, N-term HIS tag) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
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E3 SUMO-Protein Ligase NSE2 (NSMCE2) (AA 1-247) protein (His tag)
custom-made NSMCE2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
E3 SUMO-Protein Ligase NSE2 (NSMCE2) (AA 1-247) protein (His tag)custom-made NSMCE2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
E3 SUMO-Protein Ligase NSE2 (NSMCE2) (AA 1-247) protein (GST tag)NSMCE2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the N-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))
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Alternative Name
- Nsmce2
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Background
- This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance.
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Molecular Weight
- 27.8 kDa
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NCBI Accession
- NP_775956
Target
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