Quick Overview for KPNB1 Protein (DYKDDDDK Tag) (ABIN2727677)
Target
See all KPNB1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This KPNB1 protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human NTF97 (full length, C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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Karyopherin (Importin) beta 1 (KPNB1) (AA 1-876) protein (His tag)
custom-made KPNB1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Karyopherin (Importin) beta 1 (KPNB1) (AA 1-876) protein (His tag)custom-made KPNB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Karyopherin (Importin) beta 1 (KPNB1) (AA 1-876) protein (GST tag)KPNB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Karyopherin (Importin) beta 1 (KPNB1) (AA 1-876) protein (His tag)KPNB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Karyopherin (Importin) beta 1 (KPNB1) (AA 1-876), (full length) protein (His tag)KPNB1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
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Alternative Name
- Ntf97
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Background
- Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 97 kDa
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NCBI Accession
- NP_002256
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Pathways
- Protein targeting to Nucleus
Target
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