NUF2 Protein (His tag)
Quick Overview for NUF2 Protein (His tag) (ABIN2727781)
Target
See all NUF2 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This NUF2 protein is labelled with His tag.
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Characteristics
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- Recombinant human NUF2 / CDCA1 (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
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NUF2, NDC80 Kinetochore Complex Component (NUF2) (AA 1-464) protein (His tag)
custom-made NUF2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NUF2, NDC80 Kinetochore Complex Component (NUF2) (AA 1-463) protein (His tag)custom-made NUF2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NUF2, NDC80 Kinetochore Complex Component (NUF2) (AA 1-464) protein (GST tag)NUF2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the N-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))
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Alternative Name
- Nuf2,cdca1
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Background
- This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
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Molecular Weight
- 54.1 kDa
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NCBI Accession
- NP_663735
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Pathways
- Maintenance of Protein Location
Target
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