NUMB Protein (DYKDDDDK Tag)
Quick Overview for NUMB Protein (DYKDDDDK Tag) (ABIN2727805)
Target
See all NUMB ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This NUMB protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human NUMB (C-term flag tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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Numb Homolog (NUMB) (AA 1-651) protein (His tag)
custom-made NUMB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Numb Homolog (NUMB) (AA 1-653) protein (His tag)custom-made NUMB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Numb Homolog (NUMB) (AA 1-135) protein (GST tag)NUMB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Numb Homolog (NUMB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NUMB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Numb Homolog (NUMB) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NUMB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 5 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NUMB (Numb Homolog (NUMB))
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Alternative Name
- Numb
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Background
- Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 66 kDa
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NCBI Accession
- NP_001005744
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Pathways
- Cell-Cell Junction Organization
Target
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