MGMT Protein (Myc-DYKDDDDK Tag)
Quick Overview for MGMT Protein (Myc-DYKDDDDK Tag) (ABIN2727841)
Target
See all MGMT ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This MGMT protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human O-6-methylguanine-DNA methyltransferase (MGMT) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
custom-made MGMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-211) protein (His tag)custom-made MGMT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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Alternative Name
- O-6-Methylguanine-Dna Methyltransferase (Mgmt)
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Background
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 24.9 kDa
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NCBI Accession
- NP_002403
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Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Target
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