OCT4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for OCT4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2727872)
Target
See all OCT4 (POU5F1) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This OCT4 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human OCT3/4 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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POU Class 5 Homeobox 1 (POU5F1) (AA 1-164) protein (GST tag)
POU5F1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
POU Class 5 Homeobox 1 (POU5F1) proteinPOU5F1 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) protein (His tag)POU5F1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
POU Class 5 Homeobox 1 (POU5F1) (AA 233-360) protein (His-IF2DI Tag)POU5F1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming." in: Cell, Vol. 161, Issue 3, pp. 555-68, (2015) (PubMed).
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- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Alternative Name
- Oct3/4
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Background
- This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing&aposs sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
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Molecular Weight
- 38.4 kDa
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NCBI Accession
- NP_002692
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Pathways
- Stem Cell Maintenance
Target
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