OCT4 Protein (AA 1-360) (His tag)

Catalog No. ABIN7124538

This Recombinant OCT4 protein is expressed in Escherichia coli (E. coli).

Quick Overview for OCT4 Protein (AA 1-360) (His tag) (ABIN7124538)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-360

Purification tag / Conjugate: This OCT4 protein is labelled with His tag.

Purpose: Recombinant Human POU5F1

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background: Octamer binding transcription factor 4, MGC22487, Oct 3, Oct 4, Oct-3, Oct-4, OCT3, Oct4, Octamer binding protein 3, Octamer binding protein 4, Octamer binding transcription factor 3, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3

Molecular Weight: 39.5 kDa

UniProt: Q01860

Pathways: Stem Cell Maintenance