PAK4 Protein (Transcript Variant 1)
Quick Overview for PAK4 Protein (Transcript Variant 1) (ABIN2728177)
Target
See all PAK4 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human PAK4 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Specific activity was determined as 5,595 pmoles/min/μg, according to the Zlyte assay protocol
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P21-Activated Kinase 4 (PAK4) (AA 1-593) protein (His tag)
custom-made PAK4 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (His tag)custom-made PAK4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
P21-Activated Kinase 4 (PAK4) (AA 1-259) protein (His tag)PAK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (His tag)PAK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PAK4 (P21-Activated Kinase 4 (PAK4))
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Alternative Name
- Pak4
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Background
- PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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Molecular Weight
- 33.3 kDa
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NCBI Accession
- NP_005875
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Pathways
- RTK Signaling
Target
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