PAM Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728195

This Recombinant PAM protein is expressed in HEK-293 Cells.

Quick Overview for PAM Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2728195)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This PAM protein is labelled with Myc-DYKDDDDK Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human PAM (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Biological Activity Comment: The specific activity of PAM was determined by measuring the product D-Tyr-Val-NH2 formation from a conversion of D-Tyr-Val-Gly. The reaction was carried out at 37C for 60min in the buffer containing 50 mM MES, pH6.0, 1 μM CuCl2, 2000 units/ml of Catalase, 5 mM L-ascorbic acid, and 50 uM of D-Tyr-Val-Gly as the substrate

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: Pam

Background: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 94.2 kDa

NCBI Accession: NP_620176