Quick Overview for PAPSS1 Protein (Myc-DYKDDDDK Tag) (ABIN2728214)
Target
See all PAPSS1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PAPSS1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PAPSS1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-624) protein (His tag)
custom-made PAPSS1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-624) protein (His tag)custom-made PAPSS1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-603) protein (GST tag)PAPSS1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 24-624) protein (His tag)PAPSS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))
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Alternative Name
- Papss1
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Background
- Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008].
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Molecular Weight
- 70.7 kDa
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NCBI Accession
- NP_005434
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Pathways
- Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process
Target
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