PCBP2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for PCBP2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2728352)
Target
See all PCBP2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This PCBP2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PCBP2 / hnRNP-E2 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Poly(rC) Binding Protein 2 (PCBP2) (AA 1-365) protein (His tag)
custom-made PCBP2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Poly(rC) Binding Protein 2 (PCBP2) (AA 1-362) protein (His tag)custom-made PCBP2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Poly(rC) Binding Protein 2 (PCBP2) (AA 1-362) protein (GST tag)PCBP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Poly(rC) Binding Protein 2 (PCBP2) (AA 174-283) protein (GST tag)PCBP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Poly(rC) Binding Protein 2 (PCBP2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PCBP2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Poly(rC) Binding Protein 2 (PCBP2) (Transcript Variant 6) protein (Myc-DYKDDDDK Tag)PCBP2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))
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Alternative Name
- Pcbp2,hnrnp-e2
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Background
- The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 38 kDa
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NCBI Accession
- NP_114366
Target
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