Quick Overview for PDHA1 Protein (Myc-DYKDDDDK Tag) (ABIN2728547)
Target
See all PDHA1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PDHA1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PDHA1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (AA 1-390) protein (GST tag)
PDHA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (AA 30-390) protein (His-IF2DI Tag)PDHA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))
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Alternative Name
- Pdha1
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Background
- The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
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Molecular Weight
- 40.2 kDa
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NCBI Accession
- NP_000275
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Pathways
- Warburg Effect
Target
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