PKLR Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for PKLR Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2729045)
Target
See all PKLR ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This PKLR protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PKLR (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (His tag)
custom-made PKLR Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (His tag)custom-made PKLR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (GST tag)PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 20-105) (Active) protein (hFc Tag)PKLR Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) protein (His tag)PKLR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) (Active) protein (His tag)PKLR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, EAA Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
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Alternative Name
- Pklr
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Background
- The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 61.6 kDa
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NCBI Accession
- NP_000289
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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