PKLR Protein (AA 1-574) (GST tag)
-
- Target See all PKLR Proteins
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-574
-
Origin
- Human
-
Source
- Wheat germ
- Purification tag / Conjugate
- This PKLR protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PKLR (Human) Recombinant Protein (P01)
- Sequence
- MSIQENISSL QLRSWVSKSQ RDLAKSILIG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS VERLKEMIKA GMNIARLNFS HGSHEYHAES IANVREAVES FAGSPLSYRP VAIALDTKGP EIRTGILQGG PESEVELVKG SQVLVTVDPA FRTRGNANTV WVDYPNIVRV VPVGGRIYID DGLISLVVQK IGPEGLVTQV ENGGVLGSRK GVNLPGAQVD LPGLSEQDVR DLRFGVEHGV DIVFASFVRK ASDVAAVRAA LGPEGHGIKI ISKIENHEGV KRFDEILEVS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NLAGKPVVCA TQMLESMITK PRPTRAETSD VANAVLDGAD CIMLSGETAK GNFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EAAFKCCAAA IIVLTTTGRS AQLLSRYRPR AAVIAVTRSA QAARQVHLCR GVFPLLYREP PEAIWADDVD RRVQFGIESG KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LSIS
- Characteristics
- Human PKLR full-length ORF ( NP_000289.1, 1 a.a. - 574 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
-
Pyruvate Kinase, Liver and RBC (PKLR) (AA 485-574) protein (GST tag)
PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) protein (His tag)PKLR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (Strep Tag)Crystallography grade PKLR Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (Strep Tag)Crystallography grade PKLR Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
- Target
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
- Alternative Name
- PKLR (PKLR Products)
- Synonyms
- PK1 Protein, PKL Protein, PKR Protein, PKRL Protein, RPK Protein, Pklg Protein, wu:fd15e01 Protein, wu:fi37e08 Protein, pk1 Protein, PKLR Protein, Pk-1 Protein, Pk1 Protein, R-PK Protein, pklr Protein, pyruvate kinase L/R Protein, pyruvate kinase, liver and RBC Protein, pyruvate kinase, liver and RBC L homeolog Protein, pyruvate kinase liver and red blood cell Protein, pyruvate kinase PKLR-like Protein, PKLR Protein, Pklr Protein, pklr Protein, pklr.L Protein, LOC100621940 Protein
- Background
-
Full Gene Name: pyruvate kinase, liver and RBC
Synonyms: PK1,PKL,PKR,PKRL,RPK - Gene ID
- 5313
- NCBI Accession
- NM_000298
- Pathways
- Ribonucleoside Biosynthetic Process
-
