Quick Overview for PLGF Protein (Transcript Variant 1) (ABIN2729096)
Target
See all PLGF (PGF) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Placenta growth factor / PGF (transcript variant 1) protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 1-3 ng/ml, corresponding to a specific activity of 0.33 - 1 x 106 units/mg, determined by the dose dependent binding to VEGFR1-Fc chimera using a functional ELISA.
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Placenta Growth Factor (PGF) (AA 19-221) protein (Fc Tag)
PGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (Active) proteinPGF Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Placenta Growth Factor (PGF) (AA 19-158) protein (His tag)PGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Placenta Growth Factor (PGF) (AA 19-158) protein (Fc Tag)PGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (AA 19-170) protein (Fc Tag)PGF Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Placenta Growth Factor (PGF) (AA 19-170) (Active) protein (His tag)PGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) (AA 19-221) (Active) protein (Fc Tag)PGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) protein (His tag)PGF Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Placenta Growth Factor (PGF) (Active) protein (His tag)PGF Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Placenta Growth Factor (PGF) (AA 1-170) protein (His-IF2DI Tag)PGF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 5 mM citric acid, 0.15 M NaCl, 5 mM NaHPO4, pH 4.0
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PLGF (PGF) (Placenta Growth Factor (PGF))
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Alternative Name
- Placenta Growth Factor,pgf
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Background
- This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011].
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Molecular Weight
- 14.5 kDa
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NCBI Accession
- NP_002623
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Pathways
- VEGFR1 Specific Signals
Target
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