PKC zeta Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for PKC zeta Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2729630)
Target
See all PKC zeta (PRKCZ) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This PKC zeta protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PRKCZ (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (His tag)
custom-made PRKCZ Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (His tag)custom-made PRKCZ Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase C, zeta (PRKCZ) (AA 454-592) protein (His-IF2DI Tag)PRKCZ Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (GST tag)PRKCZ Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase C, zeta (PRKCZ) proteinPRKCZ Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
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Alternative Name
- Prkcz
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Background
- Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
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Molecular Weight
- 46.4 kDa
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NCBI Accession
- NP_001028753
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Pathways
- NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Target
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