PRKAG1 Protein (Catalytic Subunit, Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729786

Recombinant PRKAG1 protein expressed in HEK-293 Cells.

Quick Overview for PRKAG1 Protein (Catalytic Subunit, Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2729786)

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Catalytic Subunit, Transcript Variant 2

Purification tag / Conjugate: This PRKAG1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant 2 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molecular Weight: 28.1 kDa

NCBI Accession: NP_997626

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effect