PUMA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for PUMA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730162)
Target
See all PUMA (BBC3) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This PUMA protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PUMA (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (His tag)
custom-made BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2 Binding Component 3 (BBC3) (AA 1-261) protein (His tag)custom-made BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (His tag)custom-made BBC3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2 Binding Component 3 (BBC3) (AA 141-261) protein (His-IF2DI Tag)BBC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
BCL2 Binding Component 3 (BBC3) (AA 76-170) protein (His tag,Strep II tag)BBC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant ~95 % WB, ELISA, MS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "HER2 phosphorylates and destabilizes pro-apoptotic PUMA, leading to antagonized apoptosis in cancer cells." in: PLoS ONE, Vol. 8, Issue 11, pp. e78836, (2013) (PubMed).
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: "HER2 phosphorylates and destabilizes pro-apoptotic PUMA, leading to antagonized apoptosis in cancer cells." in: PLoS ONE, Vol. 8, Issue 11, pp. e78836, (2013) (PubMed).
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- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Alternative Name
- Puma
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Background
- This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 26.3 kDa
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NCBI Accession
- NP_001120712
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Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
Target
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