PUS10 Protein (Myc-DYKDDDDK Tag)
Quick Overview for PUS10 Protein (Myc-DYKDDDDK Tag) (ABIN2730184)
Target
See all PUS10 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PUS10 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PUS10 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Pseudouridylate Synthase 10 (PUS10) (AA 1-527) protein (His tag)
custom-made PUS10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pseudouridylate Synthase 10 (PUS10) (AA 1-529) protein (His tag)custom-made PUS10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PUS10 (Pseudouridylate Synthase 10 (PUS10))
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Alternative Name
- Pus10
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Background
- Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]
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Molecular Weight
- 60.1 kDa
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NCBI Accession
- NP_653310
Target
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