RAP1A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730472

This Recombinant RAP1A protein is expressed in HEK-293 Cells.

Quick Overview for RAP1A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2730472)

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This RAP1A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human RAP1A (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Alternative Name: Rap1a

Background: This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.

Molecular Weight: 20.8 kDa

NCBI Accession: NP_002875

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor