RAP1GAP Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730475

This Recombinant RAP1GAP protein is expressed in HEK-293 Cells.

Quick Overview for RAP1GAP Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2730475)

Target: RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This RAP1GAP protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human RAP1GAP (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))

Alternative Name: Rap1gap

Background: This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Molecular Weight: 73.2 kDa

NCBI Accession: NP_002876