RASA1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730523

This Recombinant RASA1 protein is expressed in HEK-293 Cells.

Quick Overview for RASA1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730523)

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This RASA1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human RASA1 / RasGAP (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Alternative Name: Rasa1,rasgap

Background: The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Molecular Weight: 116.2 kDa

NCBI Accession: NP_002881

Pathways: Regulation of Actin Filament Polymerization, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals